By R. J. Hay (auth.), R. J. Hay MD, MRCP, MRCPath. (eds.)
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Additional info for Advances in Topical Antifungal Therapy
Bifonazole ; 0, oxiconazole; 0, naftifine microscopic findings were negative in 21 patients (70%) on bifonazole, 8 patients (27%) on oxiconazole and 2 patients (7%) on naftifine; cultural findings were negative in 28 patients (93%), 26 patients (87%) and 14 patients (47%), respectively at the same time. The changes in clinical symptoms, i. e. the improvement of the parameters followed up, are shown in Fig. 1. All clinical symptoms were improved to almost the same extent during treatment with bifonazole and oxiconazole, while the regression of symptoms in the group treated with naftifine cream was much slower, alterations still being present at the trial's termination.
An evident therapeutic effect is thus demonstrable with regard to erythema size. It is evident that no significant differences were established in the histamine wheal test between treatment 2 h before provocation and treatment 2 and 12 h before, both with regard to wheal and erythema sizes. Wheal and erythema sizes were found to be significantly smaller - especially 15 and 30 min after histamine provocation - in the areas pretreated with bifonazole and 1% hydrocortisone as compared to the areas treated with cream base or left untreated.
After stripping, the tape was laid, adhesive side down, on a sterile glass slide covered with a 30% KOH solution. Next, the slide was gently heated until boiling by passing through a flame several times. 1 ml of a 23% watery solution of FWA. A stilbene compound "Blankophor P fliissig" produced by Bayer was used as FWA. The preparation including the stained, infected tape and slide was finally pressed between pieces of filter paper to remove excess air bubbles and fluid after which it was ready for examination under the fluorescence microscope.